Genomics and High Throughput Screening

  • Olivier Couronne, PhD
    • Director
  • Charles Karan, PhD
    • Scientific Director, High Throughput Screening 

The Genomics and High Throughput Screening Shared Resource is operated jointly by the Herbert Irving Comprehensive Cancer Center and the Judith P. Sulzberger Columbia Genome Center. The facility provides access to specialized instrumentation and technical expertise in three highly complementary service components:

  • Next-generation (Nextgen) Sequencing
  • High-Throughput Screening, High Content Microscopy (HTS/HCM)
  • Medium-throughput Molecular Screening (MTMS)
  • Single Cell Analysis Core

The JP Sulzberger Columbia Genome Center

To learn more about the Genomics and High Throughput Screening Shared Resource, including user policies, pricing, and to request a consultation, please visit the Judith P. Sulzberger Columbia Genome Center.

Visit the Judith P. Sulzberger Columbia Genome Center


Next-generation (Nextgen) Sequencing

Next-generation DNA sequencing and RNA-seq have made it possible not only to look at individual genomes, but also to rapidly compare genetic sequences among multiple genomes. As next-generation sequencing technologies have made it possible to generate high-resolution genomic data much more efficiently, researchers in many fields have turned to next-generation sequencing to identify particular features of the genome that contribute to specific phenotypes.

Instrumentation

  • Illumina HiSeq 4000
  • Illumina HiSeq 2500
  • Illumina NextSeq
  • Illumina MiSeq

For Services

For queries regarding Nextgen sequencing, please contact genome@columbia.edu.


High-Throughput Screening, High Content Microscopy (HTS/HCM)

The HTS/HCM component of the Genomics Shared Resource supports both basic and translational biomedical research by assisting investigators in the development, optimization, and execution of high-throughput assays that deliver answers to questions of scientific interest. A unique way in which these technologies are used is to perturb cellular systems to dissect their regulatory mechanisms and the mechanism of action of small-molecule and genetic perturbations.

Instrumentation

  • Automated Technology Platforms
    • PerkinElmer cell::explorer
    • HighRes Biosolutions workstation
    • Hamilton Microlab STAR
    • Beckman Coulter Echo dispensing system
  • Microplate Reading Technologies
    • PerkinElmer EnVision 2104 Multilabel Microplate Reader
    • Tecan Infinite M2000 Pro
  • High-content Microscopy Instrumentation
    • GE Healthcare IN Cell Analyzer 2000

For Services

For queries regarding high-throughput screening and high-content microscopy, please contact Ck2389@cumc.columbia.edu


Medium-throughput Molecular Screening Instrumentation

The Genomics SR provides access to dedicated state-of-the-art equipment for medium throughput biological assays. These instruments allow investigators to perform sophisticated, multiplexed measurement of gene and protein expression, allowing for direct, quantitative measurement of mRNA, miRNA, and protein abundance.

Instrumentation

  • Fluidigm BioMark HD
  • Fluidigm C1 Single-Cell Autoprep System

For Services

For queries regarding medium-throughput molecular screening, contact contact genome@columbia.edu.


Single Cell Analysis Core

The Single Cell Analysis Core provides experimental and computational support for single-cell RNA-Seq (scRNA-Seq) and single-cell ATAC-Seq (scATAC-Seq). We offer services using two scRNA-Seq platforms and an scATAC-Seq platform.

Instrumentation

  • 10x Genomics Chromium Single Cell Controller
  • Beckman-Coulter Biomek 4000
  • ThermoFisher Countess II FL Automated Cell Counter
  • Miltenyi gentleMACS Octo Dissociator

For Services

For queries regarding single cell analysis, contact genome@columbia.edu.


Locations and Contact

High-Throughput Screening

(also includes queries regarding high-content microscopy)

JP Sulzberger Columbia Genome Center

High-Throughput Screening Center

Mary Woodard Lasker Biomedical Research Building

3960 Broadway; Room 330

New York, NY 10032

Contact: ck2389@cumc.columbia.edu

 

Genome Sequencing and Analysis

JP Sulzberger Columbia Genome Center

Genome Sequencing and Analysis

Mary Woodard Lasker Biomedical Research Building

3960 Broadway, Room 208 (entrance on W. 166th St.)

New York, NY 10032

Contact: genome@columbia.edu

 

Single Cell Analysis Core

JP Sulzberger Columbia Genome Center

Genome Sequencing and Analysis

Mary Woodard Lasker Biomedical Research Building

3960 Broadway, Room 208 (entrance on W. 166th St.)

New York, NY 10032

Contact: genome@columbia.edu