The Izar research laboratory in the Herbert Irving Comprehensive Cancer Center and the Columbia Center for Translational Immunology (CCTI) is affiliated with the Program for Mathematical Genomics and the New York Genome Center. We aim to dissect interactions of cancer and immunity in the tumor development, metastasis and drug resistance using high-dimensional functional single-cell genomics and imaging. The ultimate goal of this work is to inform better selection and development of therapies to improve the lives of cancer patients.
Dissecting mechanisms of early tumorigenesis and niche-specific metastasis
While the genetic landscape of primary and metastatic cancers is now well understood, it remains unclear how early genetic events determine invasive capacity and metastasis of various cancers, including melanoma. It is also unknown why some tumors have a preference to metastasize to one organ over another. To delineate these fundamental questions, we apply single-cell genomics and imaging to defined genetic models of human and murine cancer precursors in tissue-engineered and pre-clinical in vivo models. Among metastatic niches, we are particularly interested in the brain as it is a major cause for morbidity and mortality across cancers.
Delineating resistance to immune checkpoint inhibitors in patient models
Immune checkpoint inhibitors (ICI) are exciting therapies that produce potentially durable responses in a portion of cancer patients, however, the vast majority of patients do not respond. We have previously identified a set of genes that are associated with drug resistance. We now use multi-scale genome-editing and single-cell genomics in patient-derived tumor/immune models to functionally to characterize putative drivers of resistance.
Development of tools to better understand mechanisms of T cell exclusion
T cell exclusion from the tumor microenvironment is one of the most important, yet poorly defined mechanisms of resistance to immune checkpoint inhibitors. We aim to develop new experimental and analytical tools to define drivers of T cell exclusion.
Amit Dipak Amin, PhD
Amit received his BSc, MSc and PhD in genetics at the University of Leicester. Amit did his first postdoc at the University of Kansas in epigenetics and his second postdoc in cancer biology at the Universities of Arizona and Miami. His work in drug resistance highlighted the seemingly inevitable onset of acquired resistance to targeted therapy. In his work, he has found, however, that the success of cancer immunotherapy suggests exciting treatment possibilities. On sabbatical, Amit undertook an MSc in cancer immunology at the University of Nottingham. In the Izar lab, Amit focuses on honing the immune system to seek-and-destroy cancer cells to overcome their resilience.
Simon Berhe, MD, PhD
Simon is originally from Munich and studied Medicine at the Ludwig Maximilian University of Munich. During his thesis in the lab of Mark Fleming, MD, DPhil in Boston, he was involved in dissecting the genetic background of Congenital Sideroblastic Anemia and Pediatric Myelodysplastic Syndrome. He is currently a surgical resident at Columbia with the dream of becoming a Pediatric Surgeon with special focus on Oncology. In the Izar lab, he will be involved in immune profiling various different malignant disease models including pediatric solid tumors using high-dimensional functional single-cell genomics and imaging.
Jana Biermann, PhD
Computational biologist Jana Biermann is a Postdoctoral Researcher in the Department of Medicine at Columbia University. She received her MSc in Molecular Life Sciences from Humboldt University Berlin, Germany and her PhD in Medical Sciences from the University of Gothenburg, Sweden. Her PhD research focused on computational biology and genomics in breast cancer, with a particular interest in the clonal relationship between primary tumors and metastases. In the Izar Lab, Jana studies the tumor microenvironment of melanoma brain metastases using single-cell genomics to determine brain-niche specific drivers of metastasis and immune evasion.
Lindsay Carprio, MD-PhD Candidate
Lindsay Caprio is a third year MD-PhD student at Columbia University Vagelos College of Physicians and Surgeons. During her undergraduate education at Georgetown University, she investigated mechanisms of cancer metastasis in two pediatric tumors, Neuroblastoma and Ewing Sarcoma. Currently, she is interested in researching chromosomal instability as a driver of cancer cell metastasis and resistance to targeted therapies.
Patricia Ho, MD, PhD
Patricia Ho is a third year MD-PhD student at Columbia University Vagelos College of Physicians and Surgeons. A graduate of Williams College, she previously worked at the Broad Institute and Dana Farber Cancer Institute, investigating mechanisms of drug resistance in pediatric brain cancers. Currently, she is interested in researching ways to further optimize the use of immunotherapy in the treatment of cancer.
Johannes Melms, MD
Physician-scientist Johannes Melms is a post-doctoral fellow in the Izar lab. He is interested in Cutaneous Oncology and Systems Pharmacology. Johannes uses combinatorial, multi-scale CRISPR/single-cell screens in patient models to deeply characterize drivers of immune evasion and resistance to immunotherapies in melanoma. He obtained a M.D. at the TU in Munich, Germany and has been a post-doctoral fellow in the Izar Group at Dana-Farber Cancer Institute/Harvard Medical School since 2017, and joins Ben at Columbia University for continued training.
Meri Rogava, MD, PhD
Physician-scientist Meri Rogava is a post-doctoral fellow in the Izar lab. She uses CRISPR-Cas9 screens to understand site-specific tumor/immune determinants of metastasis with a special focus on the brain and the leptomeninges. She obtained a M.D. from Tbilisi State Medical University, Georgia followed by a M.S. and Ph.D. in Immunology at the University of Bonn, Germany. Meri has been a post-doc in the Izar Group at Harvard Medical School since 2018, and joins Ben at Columbia University for continued training.
Former Lab Members
Shaolin Mei, MS
Staff Scientist (2016-2019)
Current role: Ph.D. Student at MD Anderson Cancer Center, Houston, TX
Sreeram Vallabhaneni, MS
Staff Scientist (2018-2019)
Current Role: Staff Scientist at the Laboratory for Systems Pharmacology at Harvard Medical School, Boston, MA
Parin Shah, MS
Research Technician (2014-2016)
Current role: Ph.D. Student at MD Anderson Cancer Center, Houston, TX
Bokang Rabasha, BA
Research Technician (2016-2018)
Current role: Assistant Project Coordinator at Botswana Harvard Aids Partnership
Igor Katsyv, MD, PhD (2019-2020)
Current Role: Dermatopathology Fellow, Memorial Sloan Kettering
Amber Wolabaugh, PhD Candidate (2020)
Current Role: Columbia PhD Candidate in the lab of Megan Sykes, MD
Science. 2016 Apr 8;352(6282):189-96. doi: 10.1126/science.aad0501.
Cancer Res. 2019 Dec 27. pii: canres.2330.2019. doi: 10.1158/0008-5472.CAN-19-2330. [Epub ahead of print]
Elife. 2018 Jul 11;7. pii: e31657. doi: 10.7554/eLife.31657.